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Tmem192-rfp-3xha

WebAug 2, 2024 · Lysosomes promote cellular homeostasis through macromolecular hydrolysis within their lumen and metabolic signaling by the mTORC1 kinase on their limiting membranes. Both hydrolytic and signaling... WebJun 20, 2012 · In a recent proteomic study of lysosomal proteins, we identified transmembrane protein 192 (TMEM192) as a novel lysosomal membrane protein candidate. Using specific anti-TMEM192 antibody and lysosomal markers, the lysosomal localization of TMEM192 was determined by immunofluorescence. TMEM192 shows a wide expression …

The Hippo kinase LATS2 impairs pancreatic β-cell survival in

WebTMEM192-3xHA (HA) served as a loading control. (Right) Densitometric analysis of NPC1 level normalized to TMEM192-3xHA (HA). Data are presented as the mean + s.d. of 3 independent experiments. (I) (Left) Confocal analysis of Proximity Ligation Assay (PLA) between TMEM192-3xHA and NPC1. HA-lyso GSC#9 were treated as described in (H). WebSep 1, 2024 · KP4 cells stably expressing the TMEM192-mRFP-3xHA tag (referred to as LysoTag) were treated with DMSO or MEKi for 48h prior to affinity based capture on HA-conjugated beads as previously described . ... pLJM1-TMEM192-RFP-3XHA was generated as previously described (34, 64). pMRX-IP-GFP-LC3-RFP-LC3ΔG (Addgene … redding homeschool programs https://jeffstealey.com

pLJM1-Tmem192-mRFP-3xHA Addgene 134631 product …

WebFeb 2, 2024 · A synthetic cDNA encoding TMEM192-RFP-3xHA was cloned into the pLJM1 lentiviral vector (Lim et al., 2024). TMEM192-FLAG was cloned also cloned in the pLJM1 vector by amplifying the TMEM192 cDNA using primers to append a DYKDDDK (“FLAG”) peptide to the c-terminus of the resulting protein. WebThe original EGFP was removed, and Tmem192-mRFP-3xHA was inserted at NheI and EcoRI sites into the pLJM1 backbone. Vector type Mammalian Expression, Lentiviral Selectable … WebOct 7, 2024 · Briefly, HeLa TMEM192-3xHA endogenously tagged cells that were grown to 80% confluency in 150-mm plates were washed twice with PBS, scraped into tubes, and then pelleted at 500×g for 5 min. redding homes with pool

The Hippo kinase LATS2 impairs pancreatic β-cell survival in

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Tmem192-rfp-3xha

pLJC5-Tmem192-2xFlag Addgene 102929 product information

WebApr 1, 2024 · Cells with endogenously-tagged TMEM192 and stably expressing FLAG-EEA1 are referred to as 293 EL cells, for E ndo-IP and L yso-IP. These cells were also prepared in a form that has a deletion of the APP gene (293 EL APP -/- ) and the same cells reconstituted with a lentivirus stably expressing a patient mutant form (APP Sw;T700N ) which ... WebMedicaid Managed Care Requests for Applications. Please note: the official site for NCDHHS procurements is the North Carolina Interactive Purchasing System. …

Tmem192-rfp-3xha

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Webproduct name : pLJM1-Tmem192-mRFP-3xHA catalog : 134631 more info or order : Addgene product webpage citations: 2 product information Catalog Number : 134631 … Webprk5-TMEM192-3xHA PM mutant. Plasmid #149506. Purpose. Transient expression of human TMEM192. Dileucine motifs LL23-24 and LL28-29 have been mutated to AA. Depositor. David Sabatini. Article. Adelmann et al Nature. 2024 Nov 18. pii: 10.1038/s41586. Insert. TMEM192 (TMEM192 Human) Use. Transient expression

WebSep 16, 2024 · A CAG-loxP-neomycin-Stop-loxP-FRT-TMEM192-3xHA-FRT-pA cassette incorporating full-length human TMEM192 cDNA coding sequences fused to a 3x … WebLaflamme C, McKeever P, Kumar R, Schwartz J, Kolahdouzan M, Chen C, et al.Implementation of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72. elife. 2024;8: pubmed publisher

WebTMEM192 Promoter ubc Tag / Fusion Protein HA (C terminal on insert) Cloning Information Cloning method Restriction Enzyme 5′ cloning site AgeI (not destroyed) 3′ cloning site … Web(A) Localization of Tmem192-3xHAfusionproteintolysosomes.Tmem192-3xHAandlysosomeswere detected by immunofluorescence with a ntibodies to the HA …

WebMar 29, 2024 · TMEM192 transmembrane protein 192 [ (human)] Gene ID: 202431, updated on 29-Mar-2024. Summary. Enables protein homodimerization activity. Located in several …

WebOct 26, 2024 · Tmem192-3xHA and lysosomes were detected by immunofluorescence with antibodies to the HA epitope tag and the lysosomal marker LAMP2, respectively. ... (r 2 = 0.95; Fig. 1G) and even with those obtained using the less preferable LAMP1-RFP-3xHA as the lysosomal antigen tag (r 2 = 0.95; Fig. S1C), mitigating concerns that expression of … knows everything meaningWebCity of Charlotte knows everything memeWebImmunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Save time and replace your secondary antibodies with our FlexAble Antibody Labeling Kits. redding hometown buffetWebAug 13, 2024 · HA-TMEM192-isolated lysosomes represented a highly pure fraction as determined by the absence of markers for other cellular compartments such as … redding hometown buffet menuWebDisclosed herein are methods and compositions for modulating MFSD12 expression and activity to treat diseases such as lysosomal storage diseases, including cystinosis. Also disclosed are methods of altering skin pigmentation and methods of screening for MFSD12 modulation agents. redding host lions clubWebRequests for Proposals (RFPs) and Requests for Information (RFIs) North Carolina has suspended the transition of Medicaid to managed care. Medicaid beneficiaries will get … knows fashion reviewsWeb> 无血清快速细胞冻存液. QuickFreezing-M. QuickFreezing-2*M knows few bounds