WebAug 2, 2024 · Lysosomes promote cellular homeostasis through macromolecular hydrolysis within their lumen and metabolic signaling by the mTORC1 kinase on their limiting membranes. Both hydrolytic and signaling... WebJun 20, 2012 · In a recent proteomic study of lysosomal proteins, we identified transmembrane protein 192 (TMEM192) as a novel lysosomal membrane protein candidate. Using specific anti-TMEM192 antibody and lysosomal markers, the lysosomal localization of TMEM192 was determined by immunofluorescence. TMEM192 shows a wide expression …
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WebTMEM192-3xHA (HA) served as a loading control. (Right) Densitometric analysis of NPC1 level normalized to TMEM192-3xHA (HA). Data are presented as the mean + s.d. of 3 independent experiments. (I) (Left) Confocal analysis of Proximity Ligation Assay (PLA) between TMEM192-3xHA and NPC1. HA-lyso GSC#9 were treated as described in (H). WebSep 1, 2024 · KP4 cells stably expressing the TMEM192-mRFP-3xHA tag (referred to as LysoTag) were treated with DMSO or MEKi for 48h prior to affinity based capture on HA-conjugated beads as previously described . ... pLJM1-TMEM192-RFP-3XHA was generated as previously described (34, 64). pMRX-IP-GFP-LC3-RFP-LC3ΔG (Addgene … redding homeschool programs
pLJM1-Tmem192-mRFP-3xHA Addgene 134631 product …
WebFeb 2, 2024 · A synthetic cDNA encoding TMEM192-RFP-3xHA was cloned into the pLJM1 lentiviral vector (Lim et al., 2024). TMEM192-FLAG was cloned also cloned in the pLJM1 vector by amplifying the TMEM192 cDNA using primers to append a DYKDDDK (“FLAG”) peptide to the c-terminus of the resulting protein. WebThe original EGFP was removed, and Tmem192-mRFP-3xHA was inserted at NheI and EcoRI sites into the pLJM1 backbone. Vector type Mammalian Expression, Lentiviral Selectable … WebOct 7, 2024 · Briefly, HeLa TMEM192-3xHA endogenously tagged cells that were grown to 80% confluency in 150-mm plates were washed twice with PBS, scraped into tubes, and then pelleted at 500×g for 5 min. redding homes with pool