Rag2-gfp mice

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August undefined, 2024

WebMay 30, 2006 · We recently used animals (14) carrying a GFP transgene driven by the recombination-activating gene 2 (RAG2) promoter [RAG2p-GFP transgenic (Tg) mice] to … WebApr 15, 2024 · Cx3cr1 deficiency exacerbates glomerulonephritis in female MRL/lpr mice. To directly elucidate the role of CX 3 CR1 + cells in spontaneous SLE, we backcrossed the Cx3cr1-gfp/gfp locus from B6 background (The Jackson Laboratory/JAX Stock Number 005582) to lupus-prone MRL/lpr mice (JAX Stock Number 000485). The Cx3cr1-gfp/gfp …

Rag2/Il2rg Double Knockout Taconic Biosciences

WebMay 5, 2009 · A, Single cell suspensions were prepared from Lymph nodes (LNs) from naive either RAG2 -/- or IL-23R-GFP.KI RAG2 -/- mice and IL-23R(GFP) expression was analyzed on total LNs' cells. B, IL-23R-GFP.KI mice were immunized with MOG 35-55 /CFA. On day 17, IL-23R(GFP) expression was analyzed on myeloid cell populations as indicated in histogram. WebGeneration of RAG2 knockout, GFP knock-in (RAG2-GFP) mice. (A) Design of the RAG2 knockout, GFP knock-in targeting construct. The genomic restriction endonuclease map … define patriarchal power

RAG2:GFP Knockin Mice Reveal Novel Aspects of RAG2 …

WebIn the thymus and bone marrow of heterozygous RAG2-GFP mice, as expected, GFP expression was detected in the appropriate stages of developing T and B cells. Interestingly, only a fraction of Thy-1.2 (+) cells in the Peyer's patch were found to be GFP (+) amongst the peripheral lymphoid organs. WebThese grafts are not rejected in this mouse strain because the Rag2 −/− γ c −/− mice are immunodeficient, lacking NK, T and B cells. The recovered FTOC-T cells were subcutaneously infused around the skin graft ( Figure 5D ) 4 weeks posttransplantation to determine if the T cells recognized and responded to the allogenic MRL skin grafts. WebApr 14, 2024 · In order to determine the effect of the immune system on disease progression, 1 o AMLs were transplanted into secondary (2 o) non-irradiated immunodeficient Rag2 −/− γc −/− or ... define patrick henry

Generation of RAG2 knockout, GFP knock-in (RAG2-GFP) …

WebThese NG-BAC transgenic mice express enhanced green fluorescent protein (EGFP) directed by the Rag2 promoter, and may be used in studies of B and T cell development as EGFP fluorescence reflects Rag2 protein expression. #005688 WHAT TO EXPECT A confirmation will be sent after order placement. Typically, eight or more animals are produced. WebIn RAG2-transgenic mice, it was revealed that the half-life of RAG2-GFP was 2-3 days, probably much longer than that of the endogenous RAG2 protein 30. Since the expression … fee of different iimWebMice with a knockout of either Rag1 or Rag2 have a very similar phenotype in the immune system (elimination of T and B cells), but they do not have the side effect of radiation/chemotherapy sensitivity. Rag1 and Rag2 knockout mice have essentially the same phenotype, and a knockout of either gene suffices to eliminate the adaptive immune … fee of engineering college

"WebJul 12, 2024 · C57BL/6 mice were obtained from Beijing Vital River Laboratory Animal Technology Co., Ltd., Beijing, China. NG-BAC (RAG2-GFP) transgenic reporter mice were as described (Yu et al., 1999). All animal experiments were performed under animal ethical regulations, and the study protocol was approved by the Institutional Animal Care and Use … " - Rag2-gfp mice

Rag2-gfp mice

Rag2 Gfp Tg Mice The Jackson Laboratory Bioz

WebNov 24, 2024 · Rag2−/− mice of both sexes were infected with Hh, of which a subgroup was followed by infection with Hp two weeks later. Co-infected males, but not females, had significantly higher total colitis index scores in the colon at both 10 and 21 weeks post-Hh infection (WPI) and developed more severe dysplasia at 21 WPI compared with mono-Hh ... WebProvided is an siRNA conjugate targeting FAP-positive cells, comprising: a ligand portion targeting FAP, an siRNA portion that inhibits expression of survival genes in FAP-positive cells, and a linker portion located between the ligand portion and siRNA portion. Also provided are a method for preparing the siRNA conjugate, and a pharmaceutical …

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WebTCRβ + CD4 + and CD8 + T cells from spleen, LNs, and PBCs of Rag2GFPSrsf1fl/flLckCre/+ and littermate Rag2GFP (as control) mice were measured by flow cytometry. Representative histograms show the percentages of GFP + cells from indicated populations. WebFor this purpose, we expressed human RAG1-GFP with human, mouse, or zebrafish RAG2-mCherry in 293T-CJ cells. Mouse RAG2 could also prevent the formation of punctate aggregates of human RAG1 but did so with significantly lower efficiency than human RAG2, while zebrafish RAG2 only modestly reduced human RAG1 aggregation when at a …

WebMay 12, 2024 · To confirm that these cells were thymocytes, we colonized mice expressing green fluorescent protein (GFP) from the RAG2 promoter (hereafter, RAG2-GFP mice) 14 …

WebMar 14, 2024 · Rag2 KI mice showed a significant reduction in B220 10 CD2 + pre-B cells and B220 hi CD2 + cells including immature and mature recirculating B cells (Fig. 6A and … Web结果讨论. 通过视网膜电图（ERG）和光学相干断层扫描（OCT）对RD2小鼠和野生型小鼠进行视网膜病理评价，结果显示：与野生型小鼠相比，RD2小鼠表现为视网膜的异常，同时11周龄RD2小鼠暗适应和明适应ERG中a波和b波的振幅均显著下降，其中暗适应ERG波形下降更 ...

WebAug 13, 2024 · To identify iNKT cells at an early stage of development in the thymus, we used mice that express green fluorescent protein (GFP) under the control of the recombination-activating gene 2 (Rag2) promoter via a bacterial artificial chromosome transgene (Rag2 GFP), in which GFP expression indicates the ‘age’ of cells (Boursalian et …

WebMice which lack Rag2 and Il2rg do not develop functional T, B or NK cells. Analysis of spleen cells showed no detectable NK1.1+ cells, B220+ B cells or Thy1+ T cells. A small number … fee of clat examWeb(B) FACS analysis of peripheral blood of representative recipient mice at 20 wk (N; AG) and 13 wk (PG) post-transplantation. The fluorescence intensity of GFP (marking ES cell-derived cells) is indicated on the X -axis, the fluorescence intensity of differentiation markers (myeloid: Gr-1; T-cell: CD4/CD8) is indicated on the Y -axis. fee offersWebJan 19, 2005 · We used mice with a functional RAG2/GFP fusion gene inserted into the endogenous RAG2 locus ( 21) that allows for tracking of RAG2-expressing cells by their … fee of duWebRFP mice splenocytes stained with B220 APC and CD8 APC (down) ( B ) Representative FACS plots of post-depletion samples composed of a mix of B220-, CD8-, IL18R- depleted Foxp3. GFP splenocytes and B220-, CD8- depleted Foxp3. RFP splenocytes. Numbers indicate the percentage of cells within each gate. Techniques Used: Staining Figure … define pat someone on the backWebJun 24, 2024 · Sialic acid (Sia) is considered as one of the most important biomolecules of life since its derivatives and terminal orientations on cell membranes and macromolecules play a major role in many biological and pathological processes. To date, there is only a limited number of active molecules that can selectively bind to Sia and this limitation has … fee o feesWebJun 19, 2000 · To examine regulation of RAG2 expression in vivo, we and others produced mice that carry RAG2 green fluorescent protein (RAG2-GFP) indicator genes … define pattern greyed outWebFeb 25, 2010 · (A) Rag2 −/− γ c −/− male mice received a transplant subcutaneously in the left flank of MEC1 cells with or without GFP expression (10 × 10 6 cells/mouse; n = 4, repeated twice), showing no significant difference in tumor engraftment. Tumor size was evaluated by measuring perpendicular diameters by a caliper. define pattern in manufacturing process