Phospho antibody

Author: rbkn

August undefined, 2024

WebGeneral notes. This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility. - Improved … WebTo use the phospho-specific antibodies as an assay for Raf-1 activation, the Raf-1 protein is first immunoprecipitated from cell lysates with an antibody that efficiently immunoprecipitates Raf-1. The immunoprecipitates are then washed, separated by SDS–PAGE, and transferred onto a 0.2-μm pore size nitrocellulose membrane as …

Phospho-S6 (Ser235, Ser236) Monoclonal Antibody (G.22.6)

WebThe NeuroMab™ anti-Phospho-Bad (Ser136) antibody detects endogenous levels of Bad only when phosphorylated at Ser136 (equivalent to Ser99 in human and Ser137 in rat). … WebAll synaptic proteins contain a short amino-terminal domain, which is highly conserved and phosphorylated by PKA or CaM kinase i. The phosphorylation of the amino terminal serine … diabetic carbohydrate one meal

An Overview of Phosphospecific Antibodies Thermo …

WebKey features and details. Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply. Rabbit monoclonal [EPR1899 (2)] to JAK1 … WebCheck the product webpage/datasheet for recommended antibody dilution. Note that while the membrane is blocked with nonfat dry milk, phospho-specific antibodies are often diluted and incubated with the membrane in 5% BSA. Use TBS-T rather than PBS-T in wash/incubation buffers since the phosphate ion may lead to interference.* cindy livingston toledo

NeuroMab™ Anti-Phospho-Bad (Ser136) Antibody, Clone N19659

An Overview of Phosphospecific Antibodies Thermo Fisher Scient…

WebPhospho-S6 (Ser235, Ser236) Antibody (MA5-15140) in ICC/IF. Immunofluorescence analysis of Phospho-S6 (Ser235,Ser236) was performed using 70% confluent log phase U … WebPhospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb recognizes endogenous levels of Smad2 protein when phosphorylated at Ser465 and Ser467. This … diabetic carb reference wall chartWebPhosphospecific Antibodies Transcription-Specific Antibodies Phosphorylation site-specific antibodies enable the analysis of key targets in normal and disease states, including cardiovascular disease, cancer, … diabetic carb intake chart

"WebProduct name Anti-Phospho - (Ser/Thr) Phe antibody See all Phospho - (Ser/Thr) Phe primary antibodies Description Rabbit polyclonal to Phospho - (Ser/Thr) Phe Host species … " - Phospho antibody

Phospho antibody

Phospho-S6 (Ser235, Ser236) Monoclonal Antibody (G.22.6)

WebPhosphospecific antibodies are affinity-purified rabbit polyclonal or monoclonal antibodies that are monospecific for a target protein that is phosphorylated. These antibodies provide critical insight into the investigation of complex signal transductions events. On this page … Explore our diverse products for Immunoassays including matched … Antibody fragmentation is somewhat laborious, requires optimization of … Recombinant rabbit monoclonal antibodies are developed by immunizing animals … Antibody screening, titering and isotyping are important in-process and final … WebABL1/2 (phospho-Tyr393/429) Antibody detects endogenous levels of ABL1/2 only when phosphorylated at tyrosine393/429. Form: Liquid Purification: Immunoaffinity purification Concentration: 1 mg/mL Quality Control Testing: Antibody Reactive Against Synthetic Peptide. Recommend Usage: Immunohistochemistry (1:50-1:100) Immunofluorescence …

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WebAnti-Phospho Antibody HRP-Conjugated Secondary Antibody Assay Diluent TMB One-Step Substrate Stop Solution Lysis Buffer Positive Control Sample Other Materials Required Distilled or deionized water 100 ml and 1 liter graduated cylinders Tubes to prepare sample dilutions Protease and Phosphatase inhibitors WebThe NeuroMab™ anti-Phospho-p53 (Ser15) antibody (Rodent Specific) recognizes endogenous levels of p53 protein only when phosphorylated at Ser15. Expression Host Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser15 of mouse p53 protein. Species Reactivity …

WebMonoclonal Antibody for studying Bad (Ser99) phosphate. Cited in 1 publication. Highly specific and rigorously validated in-house, Phospho-Bad (Ser136) (5D8) Mouse … WebRecombinant Anti-JAK1 (phospho Y1034 + Y1035) antibody [EPR1899 (2)] - BSA and Azide free (ab203784) Datasheet Certificate of Compliance Reviews ( 1) Submit a question References (2) $8,985.00 Product size 100 µg $905 1 mg $8,985.00 Add to basket Order now and get it on Friday April 14, 2024 Custom quote Bulk request Shipping info

WebPhospho-specific antibodies detect only the phosphorylated forms of proteins in a complex protein mixture within cells. They recognize the phosphorylated amino acids in the … WebThe NeuroMab™ anti-Phospho-NDRG1 (Thr346) antibody detects endogenous levels of NDRG1 when phosphorylated at Thr346. This antibody likely cross-reacts with other conserved phosporylation sites on NDRG1 at positions Thr356 and Thr366.

WebPhospho-mTOR (Ser2448) Antibody detects endogenous levels of mTOR only when phosphorylated at Ser2448. Species Reactivity: Human, Mouse, Rat, Monkey Species …

WebThe Phospho- (Ser/Thr) Kinase Substrate Antibody Sampler Kit provides an economical means to investigate the downstream activity of select serine/threonine kinases. The kit … cindy l lee wells fargoWebFig. 5 Forskolin treatment enhances serine phosphorylation of PCBP1 as analyzed by immunoprecipitation. A) The upper set of gel images shows the enhanced phosphorylation of PCBP1 with forskolin treatment by … cindy l kingWebEach phospho-specific antibody has been tested in three different flow cytometry buffer systems: IC Fixation and Permeabilization , Foxp3/Transcription Factor Buffer, and IC … cindy l kendrickWebThe cells are stimulated, fixed, and blocked in the same well. Phospho-specific antibodies are used to assess phosphorylation status using fluorometric (Figure 4) or colorimetric detection systems. Furthermore, the phospho-protein and total protein are simultaneously detected in the same microplate well. diabetic cardiac diet handoutWebMonoclonal Antibody for studying Bad (Ser99) phosphate. Cited in 1 publication. Highly specific and rigorously validated in-house, Phospho-Bad (Ser136) (5D8) Mouse Monoclonal Antibody (CST #5282) is ready to ship. diabetic carbs before bedWebAnti-PD1 (phospho Y248) antibody [EPR19821] See all PD1 primary antibodies Description Rabbit monoclonal [EPR19821] to PD1 (phospho Y248) Host species Rabbit Tested applications Suitable for: WB, Dot blot, … cindyllou fletcherWebFor those antibodies which are suspected to be more intensely/prominently binding, one can or advised to dilute it in 5% NFM (non-fatty milk) But there are antibodies which are very weakly... diabetic cardiomyopathy application