Web1. Prepare and autoclave/sterilize stock media. Be sure that the flask contains a stir-bar. 2. The solution must cool before adding antibiotics as the heat may inactive them. Let the flask equilibrate in the water bath set at 55-60o C for a minimum of 30 min. At this point, agar solutions should be warm enough that it won't soon solidify, but cool http://rothlab.ucdavis.edu/recipes/AntibioticStocks.shtml
UA Biophysics:Protocols:Kanamycin - OpenWetWare
WebIX. STOCK SOLUTIONS AND DILUTIONS. Consider your 10% w/v sucrose and 1M NaCl, prepared as part of module VIII and stored in the refrigerator, as stock solutions from which other solutions can be made. EXAMPLE: MAKING 1% w/v SUCROSE FROM 10% w/v STOCK: A 1% sucrose solution can be prepared from the 10% stock by 1:10 … WebKanamycin: Prepare stock solution, filter sterilize using a 0.2 µm syringe filter (B-D # 309602 - Fisher Catalog # 09-719C or equivalent), and store at 2-8°C (for 3 weeks maximum) or-20°C or lower for long-term storage. Add appropriate amount to sterile culture medium (after cooling to ~ 50oC) pcd235b101 3bhe032025r0101
Answered: 2. You need to make 500mL of 7H9(+)… bartleby
WebBlasticidin: (Thermo, R21001 ): Use 2–10 µg/mL Blasticidin for selection in mammalian cells. Prepare Blasticidin stock solutions of 5–10 mg/mL by dissolving Blasticidin in sterile water and filter sterilize the solution. Blasticidin is soluble in water and acetic acid. Aqueous stock solutions are stable for 1–2 weeks at 4°C and 6–8 ... WebTetracycline must always be wrapped in foil and stored in the dar k whether it is in stock solution, in broth, or in agar. !" Stock solutions in water must be filter sterilized through a 0.22-µm filter. Antibiotics dissolved in ethanol need not be sterilized. !" Do not use expired antibiotics to make stock solutions, as they may be labile and ... WebThis protocol describes the preparation of an Ampicillin stock solution. The concentration of this stock solution is based on the weight of anhydrous Ampicillin.. Note: Typical stock solutions for this antibiotic in selectable media are made at 50mg ml-1 and are used at a final concentration of 20-40 μg ml-1 for stringent plasmids and 100 μg ml-1 for relased … pcd2112a