WebJan 20, 2024 · Step 1: Prepare several dilutions of the BSA standard, at least 5. For example, the dilutions may be 5, 10, 25, 50, 75, and 100 micrograms of BSA per milliliter. Step 2: Add reagent (which contains... WebHow does the BCA assay work? The Bicinchoninic Acid (BCA) Protein Assay primarily relies on two reactions. Firstly, the peptide bonds in the protein sample reduce Cu2+ ions, in a temperature dependent reaction, from the copper solution to Cu+. The amount of Cu2+ reduced is proportional to the amount of protein present in the solution.

Protein determination by the bca method - Rice University

WebCollect the clear supernatant and assay immediately. Cell lysates: Commercial RIPA kits are recommended for preparation of cell lysate samples. For 2 x 106 cells, lyse cells in 0.5 mL RIPA buffer, removing DNA content. Determine the protein concentration using a BCA assay kit (BN01031) with each sample concentration being ≤ 300μg for analysis. note station chrome

BCA Colorimetric Protein Assay - Protocol - OneLab

WebBCA Enhanced Protocol assay was performed on the Modulus using the Absorbance Module and the 560 nm filter paddle. Following a 60°C incubation for 30 minutes, the samples … A stock BCA solution contains the following ingredients in a highly alkaline solution with a pH 11.25: bicinchoninic acid, sodium carbonate, sodium bicarbonate, sodium tartrate, and copper(II) sulfate pentahydrate. The BCA assay primarily relies on two reactions. First, the peptide bonds in protein reduce Cu ions from the copper(II) sulfate to Cu (a temperature dependent reaction). The amount of Cu reduce… WebA lot of assays are used in the lab to determine the concentration of protein in a sample. One of the most commonly used techniques to quantify the protein c... how to set gpu 2 as primary amd