WebOct 22, 2024 · Metformin suppressed cisplatin-mediated RAD51 upregulation by decreasing RAD51 protein stability and increasing its ubiquitination. In contrast, cisplatin … WebAug 19, 2024 · In the above studies, we used γ-H2AX as a DNA damage marker to evaluate DNA damage and repair. To directly evaluate DNA damage induced by IR in the presence of different levels of eIF3a, we performed neutral comet assay at …
Regulation of gamma-H2AX and securin contribute to apoptosis …
WebDec 9, 2024 · This modified form, termed gamma-H2AX, is easily identified with antibodies and serves as a sensitive indicator of DNA DSB formation. This review focuses on the … WebAfter blocking with 6% BSA in PBS, cells were stained with a primary antibody against dsDNA (Santa Cruz Biotechnology, HYB331-01) or phosphohistone H2AX (γ-H2AX, Ser-139; Cell Signaling Technology, 9718), followed by a goat anti-mouse IgG conjugated with Alexa Fluor 568 or 633. Nuclei were stained with DAPI in the mounting medium … highline pendant
Phosphorylated fraction of H2AX as a measurement for DNA
WebDec 31, 2024 · Results: Gamma-H2AX focusing analysis has reached the conclusion that Olaparab is not the only factor inducing double-strand breaks (DSBs), but it can affect oxaliplatin-induced DSBs of XRCC2-defatted CRC cells. Cell proliferation of XRCC2-defecient CRC cell lines was restrained by combination treatment rather than olaparib or … WebFeb 3, 2024 · Phosphorylated H2AX (γ-H2AX) is a sensitive marker for DNA double-strand breaks (DSBs), but the variability of H2AX expression in different cell and tissue types makes it difficult to interpret the meaning of … WebMay 1, 2011 · Cisplatin is a chemo-therapeutic agent that forms many different adducts with DNA, removal of some of which would require formation of a DNA double strand breaks (Bhattacharyya et al., 2000). However, the radiotherapeutic agents as X-ray were reported to induce also DNA double strand breaks directly after irradiation shown as gamma … small recruitment agencies